Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
FastDigest XapI restriction enzyme recognizes R^AATTY sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AcsI, ApoI).
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).